Blogs from Powdermill Nature Reserve

Powdermill Nature Reserve is Carnegie Museum of Natural History’s environmental research center. Located 55 miles southeast of Pittsburgh in Rector, Pennsylvania, Powdermill is a field station and laboratory where researchers do long-term studies of natural populations in western Pennsylvania. In addition to being positioned for Appalachian-specific studies in ornithology, ecology, invertebrate zoology, and botany, Powdermill is a great place to spend a fun-filled day outdoors with the family.

November 24, 2020 by wpengine

One man’s trash is another man’s weather instrument

A piece of debris was recovered by staff Friday afternoon while hiking along Powdermill Run at Carnegie Museum of Natural History’s biological research station, Powdermill Nature Reserve. What initially looked like nothing more than a pile of orange plastic garbage turned out to be something much more exciting! It was actually the remains of a weather balloon carrying a recording instrument called a radiosonde.

Powdermill Run, where the instrument was found. Thank you to Bobby Ankney, Maintenance Manager at Powdermill, for wading across the stream to recover it!

These devices are deployed by the National Weather Service in order to gather data about the upper atmosphere. A large balloon (5 feet in diameter) filled with helium or hydrogen gas carries the radiosonde upward at a rate of 1,000 feet/minute. The balloon can reach an altitude of over 20 miles before it expands (due to decreasing air pressure) to a diameter of 20-25 feet and pops. Temperatures at that height can be as cold as -130⁰F!

During its ascent, the radiosonde transmits data on temperature, pressure, humidity, and GPS location to a ground tracking antenna. GPS data indicate wind speed and direction during the flight. After the balloon pops, an orange parachute carries the spent instrument slowly to the ground, where it may be recovered and returned to the National Weather Service to be reused.

close up of weather balloon label that says "Harmless Weather Instrument"

The radiosonde we found was deployed in Pittsburgh on June 28, 2020. Pittsburgh is one of 69 stations in the contiguous United States and over 800 worldwide. Weather balloons at each station are typically deployed at the same time each day, 365 days a year. Data from these instruments are used in weather forecasting, air pollution modelling, and climate change research. While removing litter from the environment is a great thing to do anyway, in this case there was a bonus learning and recycling opportunity included. Cool!

Andrea Kautz is a Research Entomologist at Carnegie Museum of Natural History’s Powdermill Nature Reserve. Museum employees are encouraged to blog about their unique experiences and knowledge gained from working at the museum.

From Collector to Director

Figure 1: CM 50625 – Rugosa Coral. Collected by M. Graham Netting in 1912. Coral body shape has a radial symmetry.

In 1912, eight-year-old M. Graham Netting unearthed 13 coral fossils within the city limits of Louisville, Kentucky. Later, as a 22-year-old Pitt student, he donated them to the Carnegie Museum of Natural History (Figure 1). When the Great Depression cut short his graduate studies at the University of Michigan in 1929, he returned to the museum as Assistant Curator of Herpetology, and worked his way up to Curator in 1932. In 1954, six months before turning 50, he was appointed Director of the Carnegie Museum of Natural History. Along the way, the Wilkinsburg native left an astonishing legacy that includes a steady growth in scientific collections, numerous wildlife dioramas in the Halls of Wildlife, and a mid-Appalachian field research station, Powdermill Nature Reserve. Upon his retirement in 1975, the Post-Gazette noted, “Long before it was “in,” Netting saw pollution of the air and water ravaging the land.”

Albert Kollar, Collection Manager of the of Section of Invertebrate Paleontology, re-discovered young Graham Netting’s horn corals while working on a multiyear review of the Bayet Collection. Netting’s label note did not provide any evidence for the stratigraphic unit that he collected from, but more on that later.

Figure 2: Carnegie Museum of Natural History exhibit reconstruction of an Early to Middle Devonian reef, 375 – 390 MYA. The reef shows Rugosa and Tabulate corals, a spiny trilobite about 18 inches in length and several straight cephalopods. Coral tentacles (shown in white) are illustrated in feeding mode. Both Rugosa and Tabulate corals went extinct at the end of the Permian Period.

Rugose corals are often called horn corals because many species have a horn shape. Horn corals attach to the sea floor by way of a sticky tentacle that protrudes from the base or curved end of the animal. Other invertebrate animals, such as brachiopods, attached in this position are described as sessile. The coral animal or “polyp” built its skeleton from calcium carbonate, a mineral formed from Bicarbonate and Calcium ions in seawater. The polyp tentacles or feeding polyp extend out from the top of the basic body for feeding (Figure 2). When the animal died, its soft tissues would have decayed and left behind the external hard mineral skeleton that fossilized.

Netting’s Louisville coral specimens are fossilized in a different way than similar corals from the nearby Falls of the Ohio middle Devonian fossil beds. His corals are lighter and fragile to the touch, conditions which gave Albert reason to compare Netting’s fossils to similar invertebrate paleontology corals from strata within the Louisville area. Sometime during or after burial, these horn coral skeletons were replaced by silica or quartz, a process known as silicification. The mineral silica can saturate a column of seawater when the seabed is overwhelmed with a large population of sponges. Sponge skeletons are composed of silica and when they die silica is added to a column or more of seawater. Volcanic eruptions eject silica into the atmosphere that eventually settles into the sea. Again potentially adding higher amounts of silica. Whatever the cause, Albert believes Netting’s corals were collected from the fossiliferous Middle Devonian age Jeffersonville Limestone, where the “lower foot of a “conglomerate” of reworked silicified Louisville Limestone” of Upper Silurian age is known to occur with silicified coral fossils (Conkin and Conkin, 1972).

Horn and Tabulate corals thrived in shallow seas forming diversified ecological reefs from about the late Silurian Period to the beginning of the Late Devonian epoch. During the Middle Devonian epoch roughly 400 Ma to 390 Ma years ago, reefs formed in central New York, southern Ontario, central Ohio, central Iowa, western Alberta, Canada, western Australia, and in Eifel, Germany.

Figure 3: Paleogeographic Map of the Middle Devonian Period – Kentucky is well south of the equator.

Louisville, during the Devonian Period, was centered in the southern hemisphere about 40 degrees south of the equator. Because of plate tectonics, the coral beds of Louisville would travel 5,500 miles over the next 390 million years to their present-day location of 38 degrees north (Figure 3). Today, fossil outcrops in the city limits of Louisville are difficult to find.

Figure 4: Graham Netting in his twenties.

When Netting retired as Director of Carnegie Museum of Natural History, he moved to a modest house next to Powdermill Nature Reserve. A seat was saved for him each Sunday at the reserve’s weekly nature talk. In 1996, he passed away. Steve Rogers, Collections Manager for the Section of Birds, recalls sipping fresh lemonade on Netting’s back porch in 1981. According to Rogers, Netting was reflective and humble. The fossil collector who became a museum director had a habit of rubbing his chin while listening to someone speak. When asked about his legacy, Rogers replied, “He was more instrumental in forming Powdermill than anyone. He had an amazing ability to be a part of a team that got things done.”

Figure 5: Graham Netting at Retirement in 1975.

As Netting prepared to step down as director in 1975, he said, “These great collections are a natural resource to answer questions about the life of the world.” On a recent day, I saw two children jumping up and down in front of the Glacier Bear diorama in Hall of North American Wildlife on a family visit to the museum. When one of the children asked, “what’s a diorama?” I thought about Graham Netting, smiled, and encouraged their engagement with the life of the world.

Many thanks to Xianghua Sun, Carnegie Museum Library Manager, Marie Corrado, Carnegie Museum Library Clerk, Stephen Rogers, Collections Manager for the Section of Birds, and John Wenzel, Director of Powdermill Nature Reserve for help researching this post.

Joann Wilson is an Interpreter in the Education Department at Carnegie Museum of Natural History and Albert Kollar is Collections Manager for the Section of Invertebrate Paleontology. Museum employees are encouraged to blog about their unique experiences and knowledge gained from working at the museum.

A Bumble’s Blog and Bumble “Weee” Catapult Craft

Wander outside in the spring and summer and I bet you will bump into a busy bumble bee bumbling among the wildflowers. Bumble bees (Bombus sp.) are rather rotund, fuzzy bees usually with black and yellow-orange stripes. Unlike the famous honey bee that hails from Europe and Asia, most bumble bee species are native to our area. Bumble bees have small underground colonies with a loose social system, compared to that of a honey bee. While bumble bees produce honey, it is in small quantities and certainly not enough to share on an industrial scale. Still, these fuzzy bumbles play an important role as pollinators of local wildflower populations and are even adapted to pollinate certain flowers!

This fashionable bumble bee is trying to squeeze into a little pair of white breeches! Duchman’s breeches (Dicentra cucullaria) to be exact. A bumble bee’s proboscis (tongue) is long enough to reach the nectaries within the nectary spurs or “pant leg” and the bee is strong enough to push open the flower petals to collect pollen.

This bumble bee is sipping nectar from squawroot (Conopholis americana). Bumble bees and flies are the typical visitors of this parasitic plant! Fun fact: bumble bees “buzz” pollenate, which means they vibrate their bodies, effectively knocking pollen down into the flowers they visit.

Upon observing the flight of a bumble bee, I have noticed that while they are strong flyers, they are not the most graceful. Sometimes they miss their mark and land on a chunk of moss instead of the flower. However, they just take that moment to rest their wings before firing up their little motor and buzzing off into, hopefully, the next flower. Their rather clunky flight pattern gave me an idea for a fun activity to help young children learn about pollinators (and sneak in a STEM activity): The Bumble “Weee” Catapult! See below for assembly instructions:

Materials

3 pipe cleaners

Paper

Pen or pencil

Coloring supplies

Scissors

Recycled egg carton

Recycled plastic spoon

4-5 large Rubber bands

Let’s dismiss the idea of launching real bumble bees and begin building the bee puppet 😉. Pick 3 different colors of pipe cleaner. Feel free to go with traditional bee colors or mix it up!

1. For the body, twist together two pipe cleaners.

2. Wrap the twisted pipe cleaners tightly around a pen or pencil and slide it off.

3. Tuck the loose ends inward and tighten up the coils on the end you would call the head.

4. For the wings, shorten the remaining pipe cleaner by 1/3, then loop it under one of the coils of the bee’s body.

5. Adjust the pipe cleaner for equal length on either side and twist at the base.

6. Roll in the loose ends to finish forming your wings, thus completing the bumble bee.

step by step photos of creating a bee from pipe cleaners

Next, build the catapult to help your bee puppet fly. The catapult consists of half of an egg carton, 4-5 rubber bands, and a recycled spoon. Tension energy is generated when the spoon is pulled back. The arm stores that energy as potential energy. Upon release, that potential energy is transferred to the object as kinetic energy, moving the object away from the arm. Pictured is the simplest catapult design with rubber bands holding the spoon, or arm of the catapult, in place. Feel free to design something more elaborate!

photo of catapult made from egg carton, plastic spoon, and rubber bands

Finally, the bumble bee needs a flower to land in! Draw a flower of your choice on a piece of paper and color it in. Many bees are able to see UV light, which means they are able see colors and patterns invisible to the human naked eye. Some flowers have nectar guides that really stand out to bees, so feel free to draw some nectar guides, or lines that point to the center on your flower targets to help guide your bumble bee!

Feel free to create as many flowers as you like and propel your little bumble bee into as many flowers as you can. Happy pollinating!

Sara Klingensmith an educator at Powdermill Nature Reserve, Carnegie Museum of Natural History’s environmental research center. Museum employees are encouraged to blog about their unique experiences and knowledge gained from working at the museum.

How to catch 311 amphibians in 10 days

Step 1: Deploy pitfall traps across Powdermill Nature Reserve

Step 2: Get out of the way and let nature do the rest

Over the course of 10 days in June of this year, I captured 311 amphibians of 12 different species. Every day, rain or shine, I spent over four hours checking 132 pitfall traps and several more hours identifying, measuring, and weighing the day’s amphibian haul. I did a rinse and repeat of this cycle for 10 days straight. Why would anyone do all of this for what Carl Linnaeus, the father of modern taxonomy, once described as “vile animals” with “a foul odor” (Wahlgren, 2011)? Although this sentiment might still ring true for some people today, I did this because amphibians are in serious trouble—more than 30% of species are facing extinction. The threats to amphibians range from habitat losses to disease epidemics, but these are merely symptoms of the underlying cause: unnatural changes brought about by the Anthropocene. Human-induced alterations to nature are irrevocably modifying biodiversity so rapidly that species we learned about in grade school are now extinct and, if we view amphibians as sentinel organisms, then the worst is yet to come.

The Powdermill Nature Reserve is a protected site in Pennsylvania’s Allegheny Mountains where, since 1956—the year it was established by a forward-thinking herpetologist— the property has functioned in a similar way as forests did before human settlement swept across the region. In the early 1980s, scientists at the Carnegie Museum of Natural History studied the amphibian community at the Powdermill Nature Reserve and, serendipitously, established the empirical baseline necessary to study how environmental changes have affected amphibian biodiversity in the Alleghenies (Meshaka, 2009).

close up photo fo orange salamander with black stripes

Examining the results of amphibian trapping during two long ago Junes offers insight into the reserve’s value. In June 1982, 78 traps captured 262 amphibians of 11 species. In June 1983, 54 traps captured 174 amphibians of 11 species. While the species richness has not changed much since the 1980s, there has been species turnover and shifts in abundance, with some species becoming more common in the community. The Two-lined Salamander (Eurycea bislineata), for example, went from 0 captures in June of 1982 and 1983 to 7 captures this June. In terms of standardized trap nights in June (i.e., the number of traps multiplied the number of days opened), a combined rate of 0.11 amphibians per trap was detected across the two years in the 1980s, compared to a rate of 0.24 amphibians per trap this year. What could the ecological scenario be that has led to such an apparent increase in the amphibian capture rate over this 40-year period? Could trophic cascades be involved? Perhaps the protection of habitats in 1956 helped forest regeneration, and this change led to improved stream health and greater water retention later into the season via increased canopy cover. By providing better habitat and more resources for the streamside invertebrates that makeup the main prey base of forest-dwelling amphibians, such a transformed system might benefit amphibian communities indirectly. It’s also possible that some entirely different mechanism produced this result.

The species that dominated captures historically and today was the Allegheny Dusky Salamander (Desmognathus ochrophaeus), which went from 0.048 individuals per trap in June from the 1980s to a slightly increased rate this June of 0.052 individuals per trap. Interestingly, the average body size of female Allegheny Dusky Salamanders has not changed over the 40-year study period, suggesting stability in morphology despite other studies reporting salamander species either shrinking (Caruso et al., 2014) or growing (McCarthy et al., 2017) in response to warmer temperatures brought about by recent climate change. Without the founding of the Powdermill Nature Reserve and the herculean efforts of historical and modern scientists from the Carnegie Museum of Natural History, we would not be able to understand the extent that humans have impacted biodiversity, let alone the data needed to solve mysteries of the modern world.

photo of black salamander with white spots

So, when I look at a Spring Salamander (Gyrinophilus porphyriticus) or a Slimy Salamander (Plethodon glutinosis) or a Four-toed Salamander (Hemidactylus scutatum) from the Powdermill Nature Reserve, I don’t see Linnaeus’s “terrible animal” with a “ghastly color”, rather, I see profound resiliency in the face of tremendous pressure, and the power that natural history collections and protected areas hold for improving our relationship with biodiversity.

Daniel F. Hughes is the Rea Post-doctoral Fellow in the Herpetology Section at Carnegie Museum of Natural History. Museum employees are encouraged to blog about their unique experiences and knowledge gained from working at the museum.

References:

Caruso, N.M., Sears, M.W., Adams, D.C. and Lips, K.R., 2014. Widespread rapid reductions in body size of adult salamanders in response to climate change. Global Change Biology, 20: 1751–1759.

Meshaka, Jr., W.E., 2009. The terrestrial ecology of an Allegheny amphibian community: Implications for land management. The Maryland Naturalist, 50: 30–56.

McCarthy, T., Masson, P., Thieme, A., Leimgruber, P. and Gratwicke, B., 2017. The relationship between climate and adult body size in redback salamanders (Plethodon cinereus). Geo: Geography and Environment, 4: e00031.

Wahlgren, R., 2011. Carl Linnaeus and the Amphibia. Bibliotheca Herpetologica, 9: 5–37.

The Molecular Lab at Powdermill

The Molecular Lab at Powdermill is a great resource because it allows us to analyze samples in-house from field studies being conducted on the reserve.

Currently, we are extracting and amplifying insect DNA from Chimney Swift feces in order to determine the dietary composition of these declining aerial insectivores. We are also screening swabs taken from amphibians and reptiles surveyed across the reserve for the presence of pathogens such as chytrid fungus, which is decimating amphibian populations across the globe.

Another ongoing lab effort involves devising a protocol for the detection of gill lice DNA from trout stream water samples. Gill lice are parasites that attach themselves to the gills of trout. This protocol would allow us to detect the presence of the parasite from a sample of water alone, without having to catch and examine the trout directly.

Relevance and the Spirit of Research at Powdermill

three people collecting specimens from a stream

When people think of scientists doing research, they tend to think of a laboratory with microscopes, technical machinery, exotic chemicals, and a person in a white coat doing “experiments” to invent a miraculous new compound, or maybe cure a disease. We have a technical laboratory with microscopes, machines, and chemicals at Powdermill, and we enjoy that kind of work. In reality, most of our research is done outside, with living plants and animals in the field. Usually we are in muddy boots, and we are more likely to be wearing rain gear and backpacks than white coats.

Our research comes in several flavors. Because we own the land, we can invest in long-term studies that require strong continuity. Examples of this would be our studies of birds that migrate in spring and autumn along the Appalachian ridges, traversing routes from the Caribbean and South America to Pennsylvania, Canada, and the Arctic. Since these studies were initiated in 1961, we have compiled the longest continuous data set of this type for any American research institute. Another example would be our forest succession research, initiated in 2012, and intended to last several decades. These long-term studies are not likely to be undertaken by the college professor who must show results promptly for promotion and tenure, so it is important that places like Powdermill commit to them.

two people doing field research in the woods

On the flip side, we support student researchers to use Powdermill for their projects that have to be completed in a short time, between one summer and three years, depending on whether the research is for a senior thesis or a PhD. Together, the students embrace many topics across the entire diversity of biological systems: What do trout eat? How effective are birds at dispersing seeds? The work of the long-term studies can be thought of as composing a careful symphony, where student projects represent the catchy tunes coming from a dance club: each centered on a good riff, immediate and focused; then another tune, and another. Both the symphony and the dance band are important to our scientific culture, and together they demonstrate the relevance of the nature reserve.

Field stations necessarily focus on topics that occur on their landscape. Most of our work has a strong relevance to Pennsylvania in particular, but also to Appalachia and eastern North America in general. Sometimes our work in Pennsylvania connects us to a much broader audience, as the migrating birds that spend the winter in South America do. We often host researchers from other countries who view Powdermill as an exotic locale. Every year we host a series of workshops to train the next generation of scientists, and every year we have far more applicants than spaces. In the last decade, an award-winning program that sponsors Latin American guests (so that the actual cost of the program is not a barrier to applicants) has trained about 120 scientists from 10 nations who came to Powdermill to learn our research techniques. When they return to their home countries to resume their scientific careers, some of our Powdermill culture goes with them.

The visitor traffic through Powdermill presents a learning opportunity for us. Getting to know our visitors sometimes introduces us to new methods or entirely new fields of research. We expand our research interests and capacity, too.

A pleasing and unique aspect of a research career at a field station is being in tune with the pulse of the natural world. You care about when it rained last, how cold it was the previous night, and why you have seen so many porcupines this year. The late freeze hurt the beeches and spice bush, but the maples and oaks are okay. Observing a small wildflower where you did not see it last year is like finding a gemstone. Training your eye to notice when certain plants bloom or when certain animals appear becomes rewarding, like playing a favorite game. We expect hummingbirds to arrive from Mexico on April 30. On May 1, they appear at our feeders. All of these observations make each day an experience with its own reward and mystery. And being connected to the natural world makes you feel very much alive.

John Wenzel is the Director at Powdermill Nature Reserve, Carnegie Museum of Natural History’s environmental research center. Museum employees are encouraged to blog about their unique experiences and knowledge gained from working at the museum.

One man’s trash is another man’s weather instrument

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